reference : Characterization of waterborne outbreak–associated Campylobacter jejuni , Walkerton, Ontario

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/reference/a7dd9a25-4323-43a8-9546-59217de13726
Bibliographic fields
reftype Journal Article
Abstract The Walkerton, Canada, waterborne outbreak of 2000 resulted from entry of Escherichia coli O157:H7 and Campylobacter spp. from neighboring farms into the town water supply. Isolates of Campylobacter jejuni and Campylobacter coli obtained from outbreak investigations were characterized by phenotypic and genotypic methods, including heat-stable and heat-labile serotyping, phage typing, biotyping, fla–restriction fragment length polymorphism (RFLP) typing, and pulsed-field gel electrophoresis. Two main outbreak strains were identified on the basis of heat-stable serotyping and fla-RFLP typing. These strains produced a limited number of types when tested by other methods. Isolates with types indistinguishable from, or similar to, the outbreak types were found only on one farm near the town of Walkerton, whereas cattle from other farms carried a variety of Campylobacter strains with different type characteristics. Results of these analyses confirmed results from epidemiologic studies and the utility of using several different typing and subtyping methods for completely characterizing bacterial populations.
Author Clark, Clifford G.; Price, Lawrence; Ahmed, Rafiq; Woodward, David L.; Melito, Pasquale L.; Rodgers, Frank G.; Jamieson, Frances; Ciebin, Bruce; Li, Aimin; Ellis, Andrea
DOI 10.3201/eid0910.020584
ISSN 1080-6059
Issue 10
Journal Emerging Infectious Diseases
Pages 1232-1241
Title Characterization of waterborne outbreak–associated Campylobacter jejuni , Walkerton, Ontario
Volume 9
Year 2003
Bibliographic identifiers
.reference_type 0
_record_number 19049
_uuid a7dd9a25-4323-43a8-9546-59217de13726